Universal antibody purification resin: The ligand of products for the purification of general antibody are mainly Protein A and Protein G. They are usually used for the purification of polyclonal and monoclonal antibody.
Monoclonal antibody purification resin: Monoclonal antibody purification products mainly refer to Protein At Beads 4FF, Protein At Beads LX and Protein SupAt Beads, which can purify monoclonal antibodies at relatively high flow rates purified, cleaning-in-place with 0.1-0.5 M NaOH. These products are suitable for industrial customers to quickly and efficiently produce and amplify antibodies
Polyclonal antibody purification resin: PabPur SulfoLink Beads 4FF enables simple and efficient covalent immobilization of sulfhydryl containing peptides, protein and other ligands to a medium for use in affinity purification procedures. In particular, the cysteine at N or C-ter-minal antigen can be effectively coupled to the PabPur Sulfolink Beads, and then be used to purify the antibody. NHS-Activated Beads 4FF is a pre-activated agarose bead. The ligand such as protein and peptides can be coupled to it by the primary amino groups. The pre-activated medium can be used to prepare affinity adsorbents which can isolate the specific substances from complex mixtures, often achieving high purity in a single step.
The diversity of the antibody-antigen interaction and our ability to manipulate the characteristics of the interaction has created many uses for antibodies and antibody fragments, both for immunochemical techniques within general research and for therapeutic and diagnostic applications.
The use of recombinant technology opens up the potential to create an infinite number of
combinations between immunoglobulins, immunoglobulin fragments, tags and selected proteins, further manipulating these molecules to our advantage.
The advantages of using a protein/peptide tag fused to the recombinant protein to facilitate its purification and detection are now widely recognized. In some cases, tags may improve the stability and solubility of recombinant proteins.
A group-specific Affinity chromatography medium has an affinity for a group of related substances rather than for a single type of molecule. The same general ligand can be used to purify several substances (for example members of a class of enzymes) without the need to prepare a new medium for each different substance in the group. Within each group there is either
structural or functional similarity. The specificity of the Affinity chromatography medium derives from the selectivity of the ligand and the use of selective elution conditions
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